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pTHH59 [RW-24]
pTHH59 [RW-24]
規格:
貨期:
編號:B175692
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pTHH59 [RW-24]
商品貨號 B175692
Designations pTHH59 [RW-24]
Species Homo sapiens, human
Depositors RL White
Vector
Construct size (kb): 8.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 3.799999952316284
Gene product: DNA Segment, single copy [D17S4]
Insert Size (kb) 3.800
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 3.8; EcoRI--4.4, 3.8; HindIII--6.6, 1.7; BamHI--7.0, 1.2; HincII--3.8, 3.3, 1.15.
The probe detects a genomic EcoRI fragment of 8.6 kb and does not hybridize to rat or mouse sequences.
RFLPs detected at normal hybridization and stringency; washes at 0.1X SSC, 0.1% SDS, 65C.
Enzyme(s) not detecting polymorphism: BglII.
References

Leach RJ, et al. Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. Genomics 5: 167-176, 1989. PubMed: 2571568

Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050

Barker D, et al. Gene for von Recklinghausen neurofibromatosis is in the pericentromeric region of chromosome 17. Science 236: 1100-1102, 1987. PubMed: 3107130

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pTHH59) on chromosome 17 [D17S4]. Nucleic Acids Res. 16: 3598, 1988. PubMed: 2897667

Peter J O'Connell, personal communication

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