This is the only species of this genus that produces a microplasmodium.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
plant
Product Format
dried
Type Strain
no
Comments
This is the only species of this genus that produces a microplasmodium. The microplasmodium gives rise to large multinucleate cysts.
dark brown originally rich matted fibrous plant material, Riverwalk, Tucson, AZ, 1992.
Medium
ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Enterobacter aerogenes ATCC 13048 and mixed bacteria
Protocol: Once the culture is established, the cysts will excyst and the trophozoites will comsume the bacteria in the medium. When the bacterial numbers have been reduced below a certain level, the Cercomonas will once again encyst. The cysts are stabile for at least 1-2 months. Subculture by detaching the cysts from the surface of the flask with a sterile cell scrapper, agitate, and aseptically transfer a 0.1-ml aliquot to a fresh flask containing 10 ml of bacterized medium 802.
Subcultivation
Protocol: Once the culture is established, the cysts will excyst and the trophozoites will comsume the bacteria in the medium. When the bacterial numbers have been reduced below a certain level, the Cercomonas will once again encyst. The cysts are stabile for at least 1-2 months. Subculture by detaching the cysts from the surface of the flask with a sterile cell scrapper, agitate, and aseptically transfer a 0.1-ml aliquot to a fresh flask containing 10 ml of bacterized medium 802.
Name of Depositor
TK Sawyer
Chain of Custody
ATCC <<--TK Sawyer<<--T.A. Nerad
References
dark brown originally rich matted fibrous plant material, Riverwalk, Tucson, AZ, 1992
