| 產品名稱 |
SMH-5 |
| 商品貨號 |
B175182 |
| Designations |
SMH-5 |
| GenBank Number |
X83618
|
| Species |
Homo sapiens, human |
| Depositors |
FG Hegardt |
| Vector |
Construct size (kb): 5.019999980926514 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HMGCS2
Gene name: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
(mitochondrial)
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 p13-p12
Tissue: liver
Type of DNA: cDNA
Insert end: Modification: EcoRI
Insert end: Modification: EcoRI
Insert size (kb): 2.058
Cross references: DNA Seq. Acc.: X83618
Nucleotides 1-2058 of the insert correspond to
nucleotides 1-2058 of X83618. Insert lengths(kb): 2.058000087738037 Tissue: liver Gene product: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) [HMGCS2] |
| Insert Size (kb) |
2.058 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; HindIII--3.2, 0.9, 0.9; KpnI--3.2, 1.8; PstI--4.4, 0.55; StuI--5.0. The insert contains the following restriciton sites (approximate kb from the 5' end): HindIII--0.82,1.60; StuI--1.12; KpnI--1.52; PstI--1.59. Use of BLAST shows that 99% (2468/1476) of nt 485-1960 in X83618 are identical to nt 1-1474 of U12789. |
| References |
Mascaro C, et al. Molecular cloning and tissue expression of human mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase. Arch. Biochem. Biophys. 317: 385-390, 1995. PubMed: 7893153
|
