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Construct size (kb): 3.674000024795532

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR1000
Intact vector size: 3.000
Type of vector: plasmid
Vector end: EcoRI
Vector end: HindIII
Cloning sites:
Polylinker sites: ApaI KpnI SacI SpeI PacI HindIII XmaI ins site NotI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): kanR
promoter: lac, T7
replicon: ColE1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PMP22
Genomic copy number: unique
Gene name: peripheral myelin protein 22
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 p12-p11.2
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: EcoRI
Insert size (kb): 0.674
Cross references: DNA Seq. Acc.: M94048

Insert lengths(kb): 0.6740000247955322

Gene product: peripheral myelin protein 22 [PMP22]

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 0.75; BglI--3.2, 0.55; EcoRI--3.7; PstI--2.1, 1.6; SacI--3.7.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.09; BglI--0.32.

The gene appears to be located entirely within the Charcot-Marie-Tooth disease (CMT1A) duplication.

Detects genomic restriction fragments of the following sizes (kb): human, HindIII--7.4, 2.5; mouse, HindIII--5.1, 2.1; hamster, HindIII--7.5, 2.8; human, EcoRI--11, 7.9.

Corresponds to nt 147-820 of the published sequence, and contains the complete coding region (nt 50-529 of the published sequence).

Detects transcripts of 1.8, 1.3, and 0.8 kb.