This is preserved in a solution containing 10% glycerol and 0.27 M CsCl. The CsCl should be diluted to less than 40 mM before infection. About 4% hamster impurity by in situ hybridization of sorted chromosomes; about 25% hamster impurity by plaque hybridization. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 43. |
