Restriction digests of the clone give the following sizes (kb): EcoRI--2.6; HindIII--2.2, 0.4; NotI--2.23; 0.37. To be used with pVEX1211 or pVEX1212 (ATCC 77420, 77421). The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when chloramphenicol selection is imposed in a host not expressing pir protein. The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir. In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates [pVEX1211(pVEX1212) and pVEX2211(pVEX2212)] provide two loxP sites for site-specific recombination that deletes the intervening target sequence. Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages. Differs from pVEX2211 (ATCC 77422) only in the orientation of the polylinker. The order of the major features in the plasmid is: R6K ori - loxP - cmlR - NotI - EcoRI/MCS/HindIII - NotI - HindIII. |
