| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.0, 4.6; BamHI--6.6, 2.2, 2.0, 1.8, 0.9, 0.62; HindIII--7.4, 5.3, 0.65, 0.48, 0.36; PstI--7.0, 3.65, 0.96, 0.87, 0.68, 0.37, 0.22; SalI--17.0. Codominant segregation of the BamHI RFLP demonstrated in 3 families. Detects genomic restriction fragments of the following sizes (kb): owl monkey HindIII--7.4, 4.6, 2.7; mouse HindIII--10.5, 4.0. Subcloned from lambda hINS-3. Contains 5' flanking sequences, exon 1 encoding part of the 5' untranslated region of IGF2, and part of the adjacent intron 1. Allele frequencies are different (A1 = 0.79, A2 = 0.21) in a Chinese population. The presence of the extra copy of pBR322 does not appear to generate any hybridization problems because the entire plasmid has been used as a probe. The construct appears to include 2 copies of the vector with the 8.6 kb insert from lambdahINS-3 because the intensity of the 4.4 kb band in the EcoRI digest is the same as the 8.8 kb band and there is an extra band in the SalI digest. Enzyme(s) not detecting polymorphism: ApaI, BanI, BglI, BglII, DraI, KpnI, MboII, MspI, PvuII, RsaI, SstI, XbaI. |
| References |
Ma NS, et al. Homologs of eleven loci on human chromosome 11 assigned to two owl monkey chromosomes. Cytogenet. Cell Genet. 56: 206-211, 1991. PubMed: 2055119
Xiang K, et al. BamHI RFLP at the insulin-like growth factor II (IGF2) locus on chromosome 11. Nucleic Acids Res. 15: 7655, 1987. PubMed: 2889194
Bell GI, et al. Isolation of the human insulin-like growth factor genes: Insulin-like growth factor II and insulin genes are contiguous. Proc. Natl. Acad. Sci. USA 82: 6450-6454, 1985. PubMed: 3901002
Graeme I Bell, personal communication
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