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Construct size (kb): 11.77999973297119

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SstI
Vector end: SstI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: human immunodeficiency virus - 1
Gene name: genome, full-length
Contains complete coding sequence?: U
Strain: BRU
Type of DNA: cDNA
Insert end: SstI
Insert end: SstI
Insert size (kb): 9.09
Cross references:

Insert lengths(kb): 9.09000015258789

Restriction digests of the clone give the following sizes (kb): SstI--9.0, 2.7; EcoRI--6.2, 4.0, 1.0; HindIII--6.2, 3.1, 1.4, 0.64.

Contains the proviral genome from bp 33 to 9129 (numbered according to Wain-Hobson et al., 1985 (Cell 40:9-17). The insert is oriented with the 5' end closer to the EcoRI site in the polylinker of the vector.

Cloned from human immunodeficiency virus 1BRU. Contains the proviral genome from bp 33 to 9129.

Cloned from human immunodeficiency virus 1BRU. Contains the proviral genome from bp 33 to 9129.

This is a subclone of lambdaJ19.