| 產(chǎn)品名稱 |
GRK4A |
| 商品貨號 |
B174585 |
| Designations |
GRK4A |
| GenBank Number |
X75897
|
| Species |
Homo sapiens, human |
| Depositors |
M Sallese |
| Applications |
contains sequence G protein-coupled receptor kinase 2 (Drosophila)-like G protein-coupled receptor kinase 4 |
| Vector |
Construct size (kb): 3.200000047683716 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: XhoI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GPRK2L
Other gene symbols: GPRK4; GRK4
Gene name: G protein-coupled receptor kinase 2 (Drosophila)-like
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 p16.3
Tissue: brain
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: EcoRI
Insert size (kb): 0.243
Cross references: DNA Seq. Acc.: X75897
Nucleotides 1-243 of the insert correspond to
nucleotides 1-243 of X75897. Insert lengths(kb): 0.2430000007152557 Tissue: brain Gene product: G protein-coupled receptor kinase 2 (Drosophila)-like(G protein-coupled receptor kinase 4) [GRK4] Target Gene: G protein-coupled receptor kinase 2 (Drosophila)-like |
| Insert Size (kb) |
0.243 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.0, 0.2; EcoRI--3.2; BamHI--3.2; HindIII--3.2; XhoI--3.2. Insert encodes the N-terminal portion of the GRK4A isoform. It contains an in-frame 96 bp insertion following codon 17 of the isoform GRK4B sequence. |
| References |
Sallese M, et al. Two isoforms of G protein-coupled receptor kinase 4 identified by molecular cloning. Biochem. Biophys. Res. Commun. 199: 848-854, 1994. PubMed: 8135832
|
