| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--7.2; AvaI--7.2; PstI--7.2; EcoRI--7.2; HindIII--4.4, 2.8. Y-227 hybridizes to a BssHII fragment of 2.7 Mb also detected by Y-280. Y-227 hybridizes to a NotI fragment of 3 Mb which is not detected by Y-280. In HindIII digests, Y-227 hybridizes to a 2.8 kb Y-specific fragment and a smaller non-Y fragment. Each is single copy. The Y-specific band was detected in 8 of 11 46,XX males, but not in 2 46,XX hermaphrodites or 1 46,XY gonadal dysgenesis patient. Isolated from a flow-sorted human Y chromosome library enriched for DNA from the Y euchromatic portion. |
| References |
Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818
Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484
Muller U, Lalande M. A physical map of the human Y-chromosome short arm. Genomics 7: 517-523, 1990. PubMed: 1974877
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