| Insert |
DNA: genomic Insert lengths(kb): 1.799999952316284 Gene product: DNA Segment, single copy (and related walk clones (within DMD gene)) [DXS164] Alleles: N1, N2, A1, A2, B1, D1, D2, E2, G1, J1, J2, K1, O1, O2, E1, G2, K2, B2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--2.7, 1.8; EcoRI--4.6; BamHI--4.6; PstI--4.6; SmaI--4.6; consistent with deposited DNA. This construct is not stable when freeze-dried and is distributed from liquid nitrogen stock. This clone is from within the DMD locus. This clone is 54.5 kb more pter than pERT87-15. The polymorphisms detected by this probe are contained within the DMD locus. The DNA must be extensively nicked to get efficient transfer of the large fragment. |
| References |
Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877
Monaco AP, et al. Localization and cloning of Xp21 deletion breakpoints involved in muscular dystrophy. Hum. Genet. 75: 221-227, 1987. PubMed: 2881877
Louis M Kunkel, personal communication
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