The insert includes these restriction sites (nucleotide position according to the published sequence): BamHI--585. Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 0.8; HindIII--4.3; BamHI--3.7,0.6, PstI--4.3, SacI--4.3. The insert contains the complete coding sequence for mouse preproIGF-IA, including 5' and 3' untranslated sequences. preproIGF-IA is one of two processing products of a common primary transcript. The insert detects transcripts of about 0.8 (major), 1.5, and 7.0 kb. |
