| 產(chǎn)品名稱 |
pSVLfur |
| 商品貨號 |
B174279 |
| Designations |
pSVLfur |
| GenBank Number |
X17094
|
| Species |
Homo sapiens, human |
| Depositors |
WJ Van De Ven |
| Applications |
in another host, produces protein paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein) paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme |
| Vector |
Construct size (kb): 9.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSVL
Intact vector size: 4.889
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: XhoI XbaI SmaI XmaI SacI BamHI
Polylinker sites: XhoI XbaI SmaI XmaI SacI BamHI
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40 late
replicon: pMB1, SV40
terminator: SV40 late polyadenylation
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PACE
Other gene symbols: FUR
Genomic copy number: unique
Gene name: paired basic amino acid cleaving enzyme (furin, membrane
associated receptor protein)
Contains complete coding sequence?: Y
Chromosome: 15; Localization: 15 q26.1
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 4.1
Cross references: DNA Seq. Acc.: X17094 Insert lengths(kb): 4.099999904632568 Gene product: paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein)( paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme) [FUR] Target Gene: paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein) |
| Insert Size (kb) |
4.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 2.4; EcoRI--4.1, 2.7, 2.2; KpnI--3.9, 3.4, 1.8; PstI--3.1, 2.5, 2.0, 1.3; SmaI--5.8, 3.4. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.48, 2.42, 3.62; KpnI--0.58, 3.97; BamHI--1.84. Contains the complete coding sequence (nt 217-2601). Constructed by introducing an EcoRI site in the SmaI site of the vector. |
| References |
van den Ouweland AM, et al. Structural homology between the human fur gene product and the subtilisin-like protease encoded by yeast KEX2. Nucleic Acids Res. 18: 664, 1990. PubMed: 2408021
Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280
van de Ven WJ, et al. Furin is a subtilisin-like proprotein processing enzyme in higher eukaryotes. Mol. Biol. Rep. 14: 265-275, 1990. PubMed: 2094803
Wim J Van De Ven, personal communication
|
