transformation host containing window in electrophoretic karyotype to provide the means to obtain pure YAC DNA
Also ATCC medium (# 2754), YEPD containing cysteine at 40 mg/L, can be used for maintaining/culturing this strain.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
transformation host containing window in electrophoretic karyotype to provide the means to obtain pure YAC DNA
Also ATCC medium (# 2754), YEPD containing cysteine at 40 mg/L, can be used for maintaining/culturing this strain.
Medium
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 25.0°C
Protocol: plus cysteine (40 mg/L)
Subcultivation
Protocol: plus cysteine (40 mg/L)
Sequenced Data
No DNA sequencing was performed in house on this product.
Name of Depositor
L Hamer
Special Collection
NCRR Contract
Isolation
derived from YPH925
References
Hamer L, et al. Isolation of yeast artificial chromosomes free of endogenous yeast chromosomes: Construction of alternate hosts with defined karyotypic alterations. Proc. Natl. Acad. Sci. USA 92: 11706-11710, 1995. PubMed: 8524833
