This is a YE-type (yeast episomal) vector with the URA3 marker. The PstI restriction site from the URA3 gene of S. cerevisiae were removed by using oligo-directed mutagenesis. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). Restriction digests of the clone give the following sizes (kb): AvaI--4.2, 0.7; EcoRV--5.0; PstI--5.0. |
