| 產品名稱 |
HFBCP82 |
| 商品貨號 |
B174032 |
| Designations |
HFBCP82 |
| GenBank Number |
Z19581
M85918
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 4.460000038146973 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S606E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens seven in absentia
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: M85918; EST: EST02442; Primer: M13 Forward;
THC id: THC109805
DNA Seq. Acc.: Z19581; EST: EST02442
Nucleotides 1-320 of the insert correspond to
nucleotides 495-814 of Z19581 with 93% identity. Insert lengths(kb): 1.5 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: seven in absentia [sina] |
| Insert Size (kb) |
1.5 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.5; EcoRI--3.0, 1.5. EST02442 matches PIR accession A36195 with 80.84% identity and 91.48% similarity over the sequence range: 15-155. Based on the alignment with the record for murine siah-2 in EMBL accession Z19581 (2198 nt, CDS 290-1267), this clone appears to contain part of the coding region. This sequence record is for Drosophila melanogaster seven in absentia (sina). |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
|
