Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Shipping Information
Frozen
Preceptrol®
no
Genotype
Toxinotype: 0
Type Strain
no
Comments
Preparation of Clostridium difficile strain 1253 ATCC® 17858-FZ™, frozen for ease of use. Lot specific titers are available upon request.
Toxinotype 0
Ribotype 054
Presence of tcdA and tcdB genes confirmed by PCR.
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Binary toxin gene cdtB not amplified by PCR
Medium
ATCC® Medium 2107: Modified Reinforced Clostridial
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor
LS McClung
References
Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986
