| 產品名稱 |
HHCMA83 |
| 商品貨號 |
B173720 |
| Designations |
HHCMA83 |
| GenBank Number |
M78677
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 3.799999952316284 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D3S1325E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens gamma-aminobutyric acid (GABA) transporter
Chromosome: 3; Localization: 3
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M78677; EST: EST00825; Primer: M13 Forward Insert lengths(kb): 0.800000011920929 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Gene product: gamma-aminobutyric acid (GABA) transporter [GABT*] |
| Insert Size (kb) |
0.800 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8; BamHI--3.8; PstI--3.8; HindIII--3.8. EST00825 matches PIR accession A35918 with 59.25% identity and 77.77% similarity over the sequence range: 2-82. This sequence record is for rat gamma-aminobutyric acid (GABA) transporter. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Polymeropoulos MH, et al. Chromosomal distribution of 320 genes from a brain cDNA library. Nat. Genet. 4: 381-386, 1993. PubMed: 8401586
J C Venter, personal communication
|
