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Size (kb): 3.7409999370574950

Vector: pKSM712 (phagemid)

Promoters: Promoter for in vitro transcription T7lac

Construction: pKSM710, pUC4-KIXX

Marker(s):kanR

Construct size (kb): 3.740999937057495

Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: BamHI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB

expression vector

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BamHI--3.75; EcoRI--3.75; PstI--3.75.

Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--2.89; EcoRI--0.39; NcoI--0.33, 3.49; PstI--3.11; PvuI--0.51; PvuII--0.48, 2.80, 3.16.

Differs from pKSM711 (ATCC 87157) only in the orientation of the kanamycin resistance gene.

Expression vector.

Temperature: 37.0°C