| 產品名稱 |
pOM5H2 |
| 商品貨號 |
B173384 |
| Designations |
pOM5H2 |
| Species |
Tobacco mosaic virus |
| Depositors |
Y Okada |
| Vector |
Construct size (kb): 6.360000133514404 |
| Insert |
DNA: cDNA Insert lengths(kb): 2.0 Gene product: genome, partial, 3' end |
| Insert Size (kb) |
2.0 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
The insert contains internal BstNI (2), HindIII, BglI and AccI sites. Restriction digests of the clone give the following sizes (kb): PstI--4.4, 2.0; EcoRI--6.2; BamHI--6.2; HindIII--5.0, 1.4; ClaI--4.4, 2.0; SalI--6.2. The insert contains the 3' end of tobacco mosaic virus cDNA. |
| Classification |
Tobamovirus |
| References |
Meshi T, et al. Molecular cloning of the complementary DNA copies of the common and cowpea strains of tobacco mosaic virus RNA. Virology 118: 64-75, 1982.
Takamatsu N, et al. Molecular cloning and nucleotide sequences of the 30K and the coat protein cistron of TMV (tomato strain) genome. Nucleic Acids Res. 11: 3767-3778, 1983. PubMed: 6304642
|
