vector containing primer sites useful for sequencing
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.76; HindIII--3.76; PstI--3.76.
After mobilization, the plasmid can be maintained by integration into the host chromosome via homologous recombination.
Differs from pK19mob (ATCC 87096) only in the orientation of the polylinker.
Cloning vector allowing mobilization into a wide range of Gram- and Gram+ bacteria.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References
Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.
Schafer A, et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145: 69-73, 1994. PubMed: 8045426
