| Comments |
Restriction digests of the clone give the following sizes (kb): HincII--3.05, 1.9, 1.25; BamHI--4.4, 2.1; EcoRI--3.1, 1.9, 1.5; HindIII--6.5; PvuII--2.35, 2.2, 1.95. This probe was also known as D18S20. This probe should be prehybridized with total human DNA (50 ug/ml) for 2-3 hours at 42C. |
| References |
Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872
Krapcho K, et al. Isolation of mapping of a polymorphic DNA sequence pEFZ10 on chromosome 18 [D18S22]. Nucleic Acids Res. 16: 1227, 1988. PubMed: 2894012
Peter J O'Connell, personal communication
|