| 產品名稱 |
pKD136, pKD8.243A plasmids in E. coli |
| 商品貨號 |
B171974 |
| Designations |
pKD136, pKD8.243A plasmids in E. coli |
| Species |
Klebsiella pneumoniae (Schroeter) Trevisan |
| Depositors |
Purdue Univ. Res. Fndn., A Berry, Purdue Univ. Res. Fndn. |
| Applications |
produces protein 3-dehydroquinate synthase produces protein 3-dehydroshikimate dehydratase produces protein 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase produces protein protocatechuate decarboxylase produces protein transketolase |
| Vector |
Information on plasmid #1: pKD8.243A
Size (kb): Unknown
Marker: cmlR
Insert component:
Insert size (kb): unknown
Genes: 3-dehydroshikimate dehydratase, aroZ, and protocatechuate decarboxylase, aroY
Source: Klebsiella pneumoniae
DNA: genomic DNA
Vector information:
Vector: unknown
Markers: cmlR
Information on plasmid #2: pKD136
Size (kb): 15.5
Marker: ampR
Insert components (3 genes inserted):
Gene 1: 3-dehydroquinate synthase, aroB
Insert size (kb): 1.65
Source: Escherichia coli
DNA: genomic DNA
Insert ends: SphI
Gene 2: transketolase, tkt
Insert size (kb): 5.0
Source: Escherichia coli RB791
DNA: genomic DNA
Insert ends: BamHI
Gene 3: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, aroF
Insert size (kb): 2.4
Source: Escherichia coli
DNA: genomic DNA
Insert ends: EcoRI linkers
Vector information:
Vector: pBR325
Vector size (kb): 5.7
Marker: ampR |
| Media |
ATCC Medium 1065 plus ampicillin (50 mcg/ml) plus chloramphenicol (50 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Freeze-dried plasmids in E. coli |
| Comments |
Restriction digests of the plasmids in this strain give the following sizes (kb): BamHI--10.0, 5.0, 4.8, 3.4, 1.1, 0.3; EcoRI--9.2, 8.8, 4.0, 2.4; SphI--18.0, 4.4, 1.8. This strain supports a portion of the biocatalytic conversion of D-glucose into adipate (a reagent in the production of nylon). It converts D-glucose into catechol. Colonies turn brown as catechol accumulates. The tkt CDS can be recovered as a 2.4 kb EcoRI fragment. The aroF CDS can be recovered as a 5.0 kb BamHI fragment. The aroB CDS can be recovered as a 1.65 kb SphI fragment. |
| References |
Draths KM, Frost JW. Genomic direction of synthesis during plasmid-based biocatalysis. J. Am. Chem. Soc. 112: 9630-9632, 1990.
Draths KM, Frost JW. Synthesis using plasmid-based biocatalysis: plasmid assembly and 3-deoxy-D-arabino-heptulosonate production. J. Am. Chem. Soc. 112: 1657-1659, 1990.
Draths KM, Frost JW. Environmentally compatible synthesis of adipic acid from D-glucose. J. Am. Chem. Soc. 116: 399-400, 1994.
Frost JW. Enhanced production of common aromatic pathway compounds. US Patent 5,168,056 dated Dec 1 1992
Pittard J, Wallace BJ. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J. Bacteriol. 91: 1494-1508, 1966. PubMed: 5326114
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
