Restriction digests of the clone give the following sizes (kb): BglI--1.5, 1.15 (doublet), 0.75; EcoRI--2.8, 1.75; HindIII--3.9, 0.55; PvuII--2.5, 1.5, 0.5; XbaI--4.5. The insert contains the following restriction sites (nt from the 5' end): XbaI--58; HindIII--494; ApaLI--1731. The sequence from 1682 to 2061 has been used as a specific probe for SPI2.3. This fragment detects the following genomic restriction fragments (kb): HindIII--3.2 (SPI2.3). Detects a mRNA of 2.2 kb. |
