| 產品名稱 |
int-2b |
| 商品貨號 |
B171460 |
| Designations |
int-2b |
| GenBank Number |
Y00848
|
| Species |
Mus musculus, mouse |
| Depositors |
C Dickson, G Peters |
| Vector |
Construct size (kb): 5.099999904632568 |
| Insert |
DNA: genomic Insert lengths(kb): 2.099999904632568 Gene product: fibroblast growth factor-3( V-INT2 murine mammary tumor virus integration site oncogene homolog, murine mammary tumor virus integration site (v-int-2) oncogene homolog, murine mammary tumor virus integration site 2, mouse, included) [Int2] Alleles: a, b |
| Insert Size (kb) |
2.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The insert contains a BamHI, a BglII, and a PstI site. Restriction digests of the clone give the following sizes (kb): SacI--3.0, 2.4; EcoRI/PstI--2.8, 1.9, 0.3, 0.2; BamHI--5.4; XbaI--5.4; HindIII--5.4. The insert includes sequences at which the U5E, U98 (BT13B), CT4, T108 (BT11), U5A, X69B, U5F, X69A and B1 tumors show integration of mouse mammary tumor virus (MMTV). This insert maps 5' to the Int-2 coding sequences. The sequence of the 3' BamHI-SacI fragment (413 nucleotides) has been reported. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Dixon M, et al. Detection and characterization of the fibroblast growth factor-related oncoprotein INT-2. Mol. Cell. Biol. 9: 4896-4902, 1989. PubMed: 2557543
Peters G, et al. Mouse mammary tumor virus integration regions int-1 and int-2 map on different mouse chromosomes. Mol. Cell. Biol. 4: 375-378, 1984. PubMed: 6321961
Dickson C, et al. Tumorigenesis by mouse mammary tumor virus: proviral activation of a cellular gene in the common integration region int-2. Cell 37: 529-536, 1984. PubMed: 6327073
Moore R, et al. Sequence, topography, and protein coding potential of mouse int-2: a putative oncogene activated by mouse mammry tumor virus. EMBO J. 5: 919-924, 1986. PubMed: 3013624
Clive Dickson, personal communication
|
