Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
A host for plating lambda libraries or clones requiring suppression of amber mutations (i.e. Charon 4A, Charon 21A, etc.). Strain LE392 same as NRRL B-14219.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor
P Leder
References
Murray NE, et al. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol. Gen. Genet. 150: 53-61, 1977. PubMed: 319344
Leder P, et al. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher-organisms: the lambdagtWES system. Science 196: 175-177, 1977. PubMed: 322278
Brunel F, et al. Cloning of bacteriophage T5 DNA fragments in plasmid pBR322 and bacteriophage lambdagtWES. Gene 8: 53-68, 1979. PubMed: 535730
Gowda K, et al. Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites. Gene 207: 197-207, 1998. PubMed: 9511762
