| 產(chǎn)品名稱 | NE-4C |
|---|---|
| 商品貨號 | B167212 |
| Organism | Mus musculus, mouse |
| Tissue | brain, neuroectodermal |
| Cell Type | neural stem cell |
| Product Format | frozen |
| Morphology | neuroepithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | embryo |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The neuroepithelial cell lines, NE-4C (ATCC CRL-2925) and NE-GFP-4C (ATCC CRL-2926) were established from the cerebral vesicles of 9-day-old mouse embryos lacking the functional p53 genes. - |
| Antigen Expression | Sox-2, Otx-2, En-1 |
| Comments | The cells from both cell lines differentiated to neurons and astrocytes when exposed to retinoic acid. The GFP-transfected clone, NE-GFP-4C, when implanted into the forebrain of adult, new-born, and fetal mice or into the mid- and forebrain vesicles of early chick embryos was capable of developing morphologically differentiated neurons. Ref
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| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium:
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| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Note: The culture flasks should be pre-coated with15µg/mL poly-L-lysine (Sigma Cat #P-9155) at least 2 hours in advance.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended. Medium renewal: Every 2 to 3 days.
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| Cryopreservation | Freeze medium: Culture medium, 90%; DMSO,10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: 5% CO2 in air recommended
Temperature: 37°C |
| Population Doubling Time | about 12 hours |
| Name of Depositor | E Madarasz |
| References | Schlett K. et al. Retinoic acid induced neural differentiation in a neuroectodermal cell line immortalized by p53 deficiency. J. Neurosci. Res. 15;47(4):405-415 (1997) PubMed: 9057134 Demeter, K et al. Fate of cloned embryonic neuroectodermal cells implanted into the adult, newborn and embryonic forebrain. Exp. Neurol.188(2):254-267 (2004) PubMed: 15246825 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
Schlett K. et al. Retinoic acid induced neural differentiation in a neuroectodermal cell line immortalized by p53 deficiency. J. Neurosci. Res. 15;47(4):405-415 (1997) PubMed: 9057134