| 產品名稱 | SU-DHL-4 |
|---|---|
| 商品貨號 | B167211 |
| Organism | Homo sapiens, human |
| Tissue | peritoneal effusion |
| Cell Type | B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast-like |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 38 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Clinical Data | 38 years Caucasian male |
| Genes Expressed | IgG+, Kappa+, IgM-, IgA-, IgD-, Lambda-. This cell line has relatively high expression levels of Bax, Bak, AIF, and high caspase-9 activity. |
| Comments | The depositor states that this cell line is negative for Epstein-Barr Virus (EBV). This cell line has a 14;18 chromosomal translocation. This cell line has a major rearrangement in the BCL-2 gene. This cell line is negative for Candida ingestion. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Cultures can be maintained by the addition of fresh medium. An inoculum of 1.0 x 105to 2.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium renewal: every 2 to 4 days (depending on cell density) |
| Cryopreservation | Storage temperature: liquid nitrogen vapor phase Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | D5S818: 11, 12 D13S317: 11, 12 D7S820: 8, 11 D16S539: 11, 13 vWA: 18, 19 THO1: 6, 9.3 Amelogenin: X Y TPOX: 9, 11 CSF1PO: 12 |
| Name of Depositor | A Epstein |
| Year of Origin | 1976 |
| References | Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165 Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794 Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
