| 產品名稱 | Renca |
|---|---|
| 商品貨號 | B167192 |
| Organism | Mus musculus, mouse |
| Tissue | kidney |
| Cell Type | epithelial |
| Product Format | frozen |
| Morphology | epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | renal adenocarcinoma |
| Age | 6 weeks |
| Gender | male |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The Renca cell line was derived from a tumor that arose spontaneously as a renal cortical adenocarcinoma in Balb/cCr mice. |
| Clinical Data | male |
| Tumorigenic | YES |
| Comments | The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver.?Ref   Murphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766?RefSalup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425?The cells do not express transforming growth factor-beta type II receptor (TbetaR-II) 10414746.?RefEngel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746 |
| Complete Growth Medium | The base medium for this cell line is RPMI-1640 (ATCC 30-2001). To make the complete growth medium, add the following components to the base medium:
This medium is formulated for use with a 5% CO2 in air atmosphere. |
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended. Medium renewal: Every 2 to 3 days.
|
| Cryopreservation | Freeze medium: RPMI-1640 Medium, 77.5%; FBS, 15% FBS; DMSO, 7.5% Storage temperature liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Population Doubling Time | approximately 24 hours |
| Name of Depositor | R Wiltrout |
| Year of Origin | 1969 |
| References | Murphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766 Salup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425 Engel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
