| 產品名稱 |
Y79 |
| 商品貨號 |
B165985 |
| Organism |
Homo sapiens, human |
| Tissue |
eye, retina |
| Cell Type |
retinoblastoma |
| Product Format |
frozen |
| Morphology |
multicellular clusters |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
retinoblastoma |
| Age |
2.5 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Karyotype |
hypertriploidy, with abnormalities including dicentrics, breaks, pulverizations and minutes |
| Derivation |
The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation. |
| Clinical Data |
2.5 years Caucasian female The donor had a strong maternal family history of retinoblastoma. |
| Comments |
The donor had a strong maternal family history of retinoblastoma.
Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Allow aggregates to settle to the bottom of the flask. Remove supernatant and discard. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 11,12 D16S539: 13,14 D5S818: 11,12 D7S820: 8,9 THO1: 6,9.3 TPOX: 8 vWA: 15,18 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 |
| Name of Depositor |
B Gallie |
| Deposited As |
Homo sapiens |
| References |
Reid TW, et al. Characteristics of an established cell line of retinoblastoma. J. Natl. Cancer Inst. 53: 347-360, 1974. PubMed: 4135597
Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
Wong HK, Ziff EB. The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent trasactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity. J. Virol. 70: 332-340, 1996. PubMed: 8523545
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