| 產(chǎn)品名稱 |
XVI E6E6G10 |
| 商品貨號(hào) |
B165977 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody can be used to assay 1,25-dihydroxy vitamin D. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against porcine (pig) receptor for 1,25-dihydroxy vitamin D3 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against porcine (pig) receptor for 1,25-dihydroxy vitamin D3 |
| Comments |
The antibody can be used to assay 1,25-dihydroxy vitamin D. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 1 mM sodium pyruvate,
2 mM L-glutamine, 10 mM nonessential amino acids, 10 mM HEPES and 50 uM
2-mercaptoethanol, 85%; fetal bovine serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml. |
| Cryopreservation |
fetal bovine serum, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
Wisconsin Alumni Res. Fndn. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Dame MC, et al. Monoclonal antibodies to the porcine intestinal receptor for 1,25-dihydroxyvitamin D3: Interaction with distinct receptor domains. Biochemistry 25: 4523-4534, 1986. PubMed: 2429689
Dame MC, et al. Assay for 1,25-dihydroxy vitamin D in sample containing vitamin D transport protein. US Patent 4,816,417 dated Mar 28 1989
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