| 產(chǎn)品名稱(chēng) |
USASK/DSIL-LHRH-A1 |
| 商品貨號(hào) |
B165914 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the carboxy terminal end of gonadotropin releasing hormone (also called luteinizing hormone releasing hormone (LHRH) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the carboxy terminal end of gonadotropin releasing hormone (also called luteinizing hormone releasing hormone (LHRH) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with LHRH conjugated to bovine serum albumin. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. A feeder layer is required to recover the cells from a frozen ampule. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine and 50 mg/l gentamicin, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
University of Saskatchewan |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Silversides DW, et al. Monoclonal antibodies against LHRH: development and immunoactivity in vivo and in vitro. J. Reprod. Immunol. 7: 171-184, 1985. PubMed: 3884802
|
