| 產(chǎn)品名稱 |
SW948 [SW-948] |
| 商品貨號(hào) |
B165803 |
| Organism |
Homo sapiens, human |
| Tissue |
colon |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Dukes' type C, grade III, colorectal adenocarcinoma |
| Age |
81 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
female 81 years Caucasian |
| Antigen Expression |
blood type O; Rh+ |
| Oncogene |
myc +; myb +; ras +; fos +; p53 +; sis -; abl -; ros -; src - |
| Genes Expressed |
carcinoembryonic antigen (CEA) 7 ng/106 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 mL cell sonicate; keratin |
| Cellular Products |
carcinoembryonic antigen (CEA) 7 ng/10 exp6 cells/10 days; colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate; keratin |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments |
The cells are positive for keratin by immunoperoxidase staining.
The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes.
N-myc and sis oncogene expression were not detected. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:15 is recommended
Medium Renewal: 1 to 2 times per week
|
| Culture Conditions |
Atmosphere: air, 100% |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 10,11 D16S539: 11,12 D5S818: 11 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 16,18 |
| Isoenzymes |
ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor |
A Leibovitz |
| Deposited As |
Homo sapiens |
| References |
Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066
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