| 產品名稱 |
SW837 [SW-837] |
| 商品貨號 |
B165802 |
| Organism |
Homo sapiens, human |
| Tissue |
rectum |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
grade IV, adenocarcinoma |
| Age |
53 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
This cell line may be used as a transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
53 years Caucasian male |
| Antigen Expression |
HLA A23, A32, B15, B35; blood type O; Rh+ |
| Genes Expressed |
carcinoembryonic antigen (CEA) 523 ng/10 6 cells/10 days; keratin |
| Cellular Products |
carcinoembryonic antigen (CEA) 523 ng/10 exp6 cells/10 days; keratin |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments |
The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative.
There is a C -> T mutation in codon 248 of the p53 gene resulting in an Arg -> Trp substitution.
The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37°C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 1 to 2 times per week
|
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 100% |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 9,12 THO1: 9.3 TPOX: 8,9 vWA: 15,16 |
| Isoenzymes |
ES-D, 1 G6PD, B PGD, A PGM1, 1 PGM3, 1 |
| Name of Depositor |
A Leibovitz |
| Deposited As |
Homo sapiens |
| References |
Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615
Chen TR, et al. Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93-117, 1982. PubMed: 7104989
Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845
Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228
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