| 產(chǎn)品名稱(chēng) |
SK-NEP-1 |
| 商品貨號(hào) |
B165716 |
| Organism |
Homo sapiens, human |
| Tissue |
kidney; Derived from metastatic site: pleural effusion
|
| Product Format |
frozen |
| Morphology |
rounded |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
25 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
(P12) hypotriploid to hypertriploid (+A1, +A2, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, secondary constrictions and large subtelocentric markers |
| Clinical Data |
25 years Caucasian female |
| Antigen Expression |
Antigen expression: Blood Type A; Rh+ |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice; forms tumor with small cells consistent with Wilms' tumor |
| Comments |
Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 105 cells/mL and maintain at between 105 and 106 cells/mL.
Medium Renewal: Every 2 to 4 days
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,10 THO1: 8,9.3 TPOX: 8,11 vWA: 15,19 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
G Trempe, LJ Old |
| Deposited As |
Homo sapiens |
| References |
Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
Orkin SH, et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-174, 1984. PubMed: 6325937
|
