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S49.1H.1AG.6/2

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編號:B165652

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產品名稱	S49.1H.1AG.6/2
商品貨號	B165652
Organism	Mus musculus, mouse
Cell Type	T lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphoma
Strain	BALB/c
Applications	The cells are resistant to 0.01 mM cortisol and 1 mM 8-azaguanine. The line was derived from S49.1 (ATCC TIB-28) by selection for resistance to cortisol and 8-azaguanine. Tested and found negative for ectromelia virus (mousepox).
Derivation	The line was derived from S49.1 (ATCC TIB-28) by selection for resistance to cortisol and 8-azaguanine.
Antigen Expression	H-2d; Thy-1.2; TL
Genes Expressed	H-2d; Thy-1.2; TL
Comments	The cells are resistant to 0.01 mM cortisol and 1 mM 8-azaguanine. The line was derived from S49.1 (ATCC TIB-28) by selection for resistance to cortisol and 8-azaguanine. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and maintain between 3 X 10 exp5 and 7 X 10 exp5 cells/ml.
Name of Depositor	M Cohn
Deposited As	Mus musculus
References	Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928 Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Schiller JG, et al. Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230

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