| 產(chǎn)品名稱 |
RPMI-7951 |
| 商品貨號(hào) |
B165631 |
| Organism |
Homo sapiens, human |
| Tissue |
skin; derived from metastatic site: lymph node |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
malignant melanoma |
| Age |
18 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number= 49; range = 47 to 66.
This is a hyperdiploid human cell line with the modal chromosome number of 49, occurring in 24% of cells. Polyploid cells occurred at 22%, which is high. Seven marker chromosomes were common to most cells, including t(10p14q); t(15q17p), t(Xp8p), del(17) (p13.1) and three others. Five others occurred only in some cells. Normal X chromosomes were absent. N14, N17 and N22 were mostly single-copied and N2 had three copies. Fluorescence examination did not show the presence of any Y-like chromosome. |
| Clinical Data |
18 years Caucasian female |
| Antigen Expression |
Antigen expression: Blood Type A; Rh+ |
| Genes Expressed |
Blood Type A; Rh+ |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice; forms pigmented melanoma |
| Comments |
A contaminant identified as Mycoplasma fermentans was eliminated in 1975. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 11,12 D16S539: 11,12 D5S818: 11 D7S820: 11,12 THO1: 9,9.3 TPOX: 8 vWA: 17,19 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
G Moore |
| Deposited As |
Homo sapiens |
| Year of Origin |
1971 |
| References |
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
J. Natl. Cancer Inst. 59: 301-307, 1977.
Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479
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