人妻少妇偷人精品无码丨色婷婷av久久久久久久丨欧美xxxx做受性欧美88丨欧洲女人牲交视频免费丨亚洲精品久久久av无码专区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > P3X63Ag8
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
P3X63Ag8
P3X63Ag8
規格:
貨期:
編號:B165457
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 P3X63Ag8
商品貨號 B165457
Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease plasmacytoma; myeloma
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Karyotype hyperdiploid; modal number = 65
Derivation
This line was derived from the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody
Cellular Products
immunoglobulin; monoclonal antibody
Comments

The line is resistant to 0.1 mM 8-azaguanine and die in HAT medium.

The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions Temperature: 37°C
Atmosphere: 5% CO2
Isotype IgG1; kappa light chain
Name of Depositor G Kohler, C Milstein
Deposited As Mus musculus
References

Koprowski H, et al. Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc. Natl. Acad. Sci. USA 74: 2985-2988, 1977. PubMed: 268647

Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497, 1975. PubMed: 1172191

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kohler G, et al. Fusion of T and B cells. Somatic Cell Genet. 3: 303-312, 1977. PubMed: 305123

Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377

Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851

Lemke H, et al. Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature 271: 249-251, 1978. PubMed: 74805

Becker M, et al., The specificity of cellular immunity: studies in guinea pigs using defined tetrapeptides containing p-azobenzenearsonate-L-tyrosine. Eur. J. Immunol. 3: 135-140, 1973.

Secher DS, et al. Spontaneous mutation in tissue culture-chemical nature of variant immunoglobulin from mutant clones of MOPC 21. FEBS Lett. 37: 311-316, 1973. PubMed: 4763339

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 中文字幕乱码一区二区免费| 亚洲精品久久区二区三区蜜桃臀| 西西人体大胆4444www| av蓝导航精品导航| 亚洲一本一道一区二区三区| 97人妻天天摸天天爽天天| 99久久人妻精品免费二区| 自愉自愉产区二十四区| 国产白丝精品爽爽久久蜜臀| 亚洲图欧洲图自拍另类高清| 99热精国产这里只有精品| 国产精品水嫩水嫩| 一本一本久久a久久精品综合| 无码人妻精品一区二区三区不卡| 国产午夜亚洲精品一区| 又粗又长又硬义又黄又爽| 亚洲精品一区二区久| 亚洲妇女行蜜桃av网网站| 本道久久综合无码中文字幕| 亚洲精品一区久久久久久| 猫咪www免费人成人入口| 少妇熟女视频一区二区三区| 久久亚洲精品无码爱剪辑| 中国熟妇内谢69xxxxx| 久久久噜噜噜www成人网| 亚洲 精品 综合 精品 自拍| 免费人成视频网站在线观看18| 18禁无遮挡羞羞啪啪免费网站| 亚洲国产一区二区a毛片| 色综合欧美在线视频区| 黑人猛挺进小莹的体内视频| 天堂在线最新版www中文| 国产精品天天狠天天看| 国产精品久久国产精品99 gif| 中文字幕av久久激情亚洲精品| 国产精品人成视频免费国产| 激情综合婷婷丁香五月| 亚洲日韩av无码一区二区三区| 四虎亚洲精品高清在线观看| 丰满少妇熟女高潮流白浆| 日产一区日产2区|