| 產品名稱 |
OM 3-1.1 |
| 商品貨號 |
B165439 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified human alpha-fetoprotein. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human alpha fetoprotein (AFP, alpha-fetoprotein) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human alpha fetoprotein (AFP, alpha-fetoprotein) |
| Comments |
Animals were immunized with purified human alpha-fetoprotein. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.005 mM 2-mercaptoethanol and HAT, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
JW Pickering |
| Deposited As |
mouse (B cell); mouse (myeloma) |
