| 產品名稱 |
OKT 9 |
| 商品貨號 |
B165438 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia,Leukemia |
| Applications |
The antibody reacts primarily with immature thymic lymphocytes and their leukemias and precipitates a 180000 dalton glycoprotein which appears to be the T cell transferrin receptor. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.U1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human T cell (activated human T lymphocyte); reacts with transferrin receptor |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human T cell (activated human T lymphocyte); reacts with transferrin receptor |
| Comments |
Animals were immunized with human T cell leukemia cells (acute lymphoblastic leukemia). Spleen cells were fused with P3X63Ag8.U1 myeloma cells. The antibody reacts primarily with immature thymic lymphocytes and their leukemias and precipitates a 180000 dalton glycoprotein which appears to be the T cell transferrin receptor. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
Ortho Pharmaceutical Corporation |
| U.S. Patent Number |
|
| References |
Kung PC, Goldstein G. Monoclonal antibody to a human early thymocyte antigen and methods of preparing same. US Patent 4,364,934 dated Dec 21 1982
Sutherland R, et al. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation- associated receptor for transferrin. Proc. Natl. Acad. Sci. USA 78: 4515-4519, 1981. PubMed: 6270680
|
