| 產品名稱 |
NCI-H1755 [H1755] |
| 商品貨號 |
B165283 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: liver |
| Product Format |
frozen |
| Morphology |
Epithelial-like and/or rounded |
| Culture Properties |
Adherent, with single cells and small clusters in suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage 4, adenocarcinoma; non-small cell lung cancer |
| Age |
65 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Derivation |
The line was established in October 1987. |
| Clinical Data |
65 years
Caucasian
female
The patient was a smoker.
60 pack years. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters and attached epithelial-like cells. Attached cells can be subcultured using 0.25% Trypsin-0.53 mM EDTA. Cultures may lose viability when the aggregates are broken down. Accordingly, cell counts are not performed while expanding the CRL-5892™ cell line.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation |
Complete culture medium, 50%; FBS, 40%; DMSO, 10% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 10,12 D16S539: 11,12 D5S818: 11,12 D7S820: 12 F13A01: 4,6 F13B: 8,10 FESFPS: 11 LPL: 12 THO1: 7 TPOX: 8 vWA: 18 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1987 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
