| 產品名稱 |
NCI-H1672 [H1672] |
| 商品貨號 |
B165277 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Cell Type |
lymphoblast |
| Product Format |
frozen |
| Culture Properties |
suspension, multicell aggregates |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage L,carcinoma; classic small cell lung cancer |
| Age |
58 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was established in July 1987. |
| Clinical Data |
The patient was a smoker.
male
Caucasian
58 years |
| Comments |
A lymphoblastoid line from the same patient is available as ATCC CRL-5959. |
| Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
| Cryopreservation |
Freeze medium:
Complete growth medium supplemented with an additional 15% fetal bovine serum and 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,11 D13S317: 11 D16S539: 11 D5S818: 11,13 D7S820: 10 THO1: 10 TPOX: 11 vWA: 17 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
July, 1987 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
