lung; derived from metastatic site: pleural effusion
Product Format
frozen
Morphology
epithelial (many rounded cells)
Culture Properties
loosely adherent
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
adenocarcinoma; bronchoalveolar carcinoma
Age
50 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established in June 1987.
Clinical Data
50 years
Caucasian
female
The patient received prior radiation therapy.
The tissue donor was a non-smoker.
Complete Growth Medium
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.
Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% fetal bovine serum and 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 15
D16S539: 13
D5S818: 10
D7S820: 8,11
THO1: 8,9.3
TPOX: 11
vWA: 16,17
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
June 1987
References
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455
