| 產品名稱 |
NCI-H1573 [H1573] |
| 商品貨號 |
B165269 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: soft tissue |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
adenocarcinoma (stage 4) |
| Age |
35 years |
| Gender |
Female |
| Ethnicity |
Caucasian |
| Derivation |
The line was established in December 1986. |
| Clinical Data |
35 years
Caucasian
female
The patient received prior radiation therapy.
The patient was a smoker.
15 pack years. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
- Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to culture vessels.
- Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended. |
| Cryopreservation |
Freeze medium: complete culture medium described above supplemented with 7.5% (v/v) DMSO. |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X CSF1PO: 10,12 D13S317: 11,13 D16S539: 12 D5S818: 11,13 D7S820: 9,11 THO1: 6 TPOX: 8,11 vWA: 17,18 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1986 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
