| 產(chǎn)品名稱(chēng) |
NCI-H1355 [H1355] |
| 商品貨號(hào) |
B165258 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: pleural effusion |
| Product Format |
frozen |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage 4, adenocarcinoma |
| Age |
53 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 54; range = 43-66 |
| Derivation |
The cell line was established in March 1986 from pleural effusion metastasis of lung. |
| Clinical Data |
53 years Caucasian male |
| Complete Growth Medium |
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing |
Cultures can be maintained by the addition of fresh medium or by replacement of medium. Alternatively, cultures can be established by centrifugation and subsequent resuspension of the pellet. The cells grow in floating aggregates of round cell clusters.
Medium Renewal: twice weekly
Note: Add fresh medium as the cell density increases. |
| Cryopreservation |
Freeze medium: Complete culture medium, 85%; fetal bovine serum, 10%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 11,13
D7S820: 12
THO1: 9
TPOX: 9,12
vWA: 14,17 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1986 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
