| 產品名稱 |
N22 |
| 商品貨號 |
B165221 |
| Organism |
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a monomorphic determinant on the I-A and I-E region products. |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse class II major histocompatibility antigens (monomorphic I-A, I-E) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse class II major histocompatibility antigens (monomorphic I-A, I-E) |
| Comments |
Animals were immunized with mouse dendritic cells. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with a monomorphic determinant on the I-A and I-E region products. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, supplemented with 50 ?M 2-mercaptoethanol, 90-95%; fetal bovine
serum, 5-10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG |
| Name of Depositor |
RM Steinman |
| Deposited As |
hamster, Armenian (B cell); mouse (myeloma) |
| References |
Metlay JP, et al. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. PubMed: 2185332
|
