| 產品名稱 |
mSXL 18 |
| 商品貨號 |
B165201 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with epitopes expressed on the female specific sex lethal gene product. The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras. The antibody cross-reacts with antigens present in extracts of D. melanogaster testes, HeLa cells and the common house fly (Musca domestica). It binds Protein A and is useful for immunoprecipitation assays and tissue staining in whole mount embryo and ovaries. |
| Derivation |
Animals were immunized with purified LacZ - Sxl fusion protein. Spleen cells were fused with Sp2/0 myeloma cells. |
| Clinical Data |
The antibody reacts with epitopes expressed on the female specific sex lethal gene product. The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the female specific sex lethal gene product of Drosophila melanogaster (Sxl) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the female specific sex lethal gene product of Drosophila melanogaster (Sxl) |
| Comments |
Animals were immunized with purified LacZ - Sxl fusion protein. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with epitopes expressed on the female specific sex lethal gene product. The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras. The antibody cross-reacts with antigens present in extracts of D. melanogaster testes, HeLa cells and the common house fly (Musca domestica). It binds Protein A and is useful for immunoprecipitation assays and tissue staining in whole mount embryo and ovaries. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: 1 to 2 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
D Bopp, P Schedl |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Bopp D, et al. Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster. Genes Dev. 5: 403-415, 1991. PubMed: 1900493
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