| 產(chǎn)品名稱 |
MES-SA |
| 商品貨號 |
B165145 |
| Organism |
Homo sapiens, human |
| Tissue |
uterus |
| Cell Type |
Epithelial |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
uterine sarcoma |
| Age |
56 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This is a hypo-diploid human cell line with the modal chromosome number of 45 in 48% of cells examined. The rate of polyploidy was 2.7%. There were five or six marker chromosomes present in each cell. Whereas t(5q6p), 10q+, and 14q+ markers were common in all cells examined, the chromosome combination of either double copy N21 lacking M5 (66.7%) or t(21qter--->C--->?)(M5)/single copy N21/minute metacentric (33.3%) distinguishes the two coexisting clones. Both X chromosomes were normal. |
| Derivation |
The MES-SA cell line was established from a surgical tumor specimen obtained at the time of hysterectomy.
Initially, the cells were grown in soft agar, and later they were transferred to multiwell plates.
|
| Clinical Data |
56 years Caucasian female |
| Tumorigenic |
Yes |
| Effects |
Yes, readily form colonies in soft agar. Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments |
The tumor was described as a poorly differentiated uterine sarcoma.
The nonepithelial origin of the cells was supported by ultrastructural studies and the absence of staining for mucin.
The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin.
They are resistant to vinblastine, dacarbazine, cisplatin, melphalan, vincristine, methotrexate and etoposide.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 mL double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Note: Newborn calf serum may be substituted for fetal bovine serum. |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11 D13S317: 11,13 D16S539: 11,12 D5S818: 13 D7S820: 7,11 THO1: 6 TPOX: 8,11 vWA: 18 |
| Population Doubling Time |
22 to 24 hrs |
| Name of Depositor |
BI Sikic |
| Deposited As |
Homo sapiens |
| Year of Origin |
1980 |
| References |
Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344
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