| 產品名稱 |
M1/22.25.8.HL |
| 商品貨號 |
B165035 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Teratocarcinoma |
| Strain |
DA |
| Applications |
The antibody reacts with pre-implantation mouse embryos, testicular cells, spermatozoa, cells in the brain and kidney and 2% of cells in the spleen. Forssman antigen is a heat stable glycolipid which is present in the cells of the early mouse embryo, teratocarcinoma stem cells, erythroblasts and sheep red blood cells but not mouse red blood cells. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Rats were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells.
Forssman antigen is a heat stable glycolipid which is present in the cells of the early mouse embryo, teratocarcinoma stem cells, erythroblasts and sheep red blood cells but not mouse red blood cells. The antibody reacts with pre-implantation mouse embryos, testicular cells, spermatozoa, cells in the brain and kidney and 2% of cells in the spleen.
Tested and found negative for ectromelia virus (mousepox). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Forssman antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Forssman antigen |
| Comments |
Rats were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells.
Forssman antigen is a heat stable glycolipid which is present in the cells of the early mouse embryo, teratocarcinoma stem cells, erythroblasts and sheep red blood cells but not mouse red blood cells. The antibody reacts with pre-implantation mouse embryos, testicular cells, spermatozoa, cells in the brain and kidney and 2% of cells in the spleen.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium.
Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
rat IgM |
| Name of Depositor |
TA Springer |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Springer T, et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539-551, 1978. PubMed: 81133
Stern PL, et al. Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells. Cell 14: 775-783, 1978. PubMed: 567532
Springer TACell -surface differentiation in the mouse: Characterization of Jumping and Lineage antigens using xenogeneic rat monoclonal antibodiesIn: Springer TAMonoclonal AntibodiesNew YorkPlenum Presspp. 185-217, 1980
enriched for T lymphocytes
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