| 產(chǎn)品名稱 |
LS411N |
| 商品貨號(hào) |
B165012 |
| Organism |
Homo sapiens, human |
| Tissue |
cecum |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Dukes' type B, colorectal carcinoma |
| Age |
32 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 75, X.
|
| Derivation |
LS411N is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy of a Dukes' B, poorly differentiated cecal carcinoma. The cells obtained after passage in nude mice are referred to as LS411N cells. |
| Clinical Data |
32 years
Caucasian
male
|
| Antigen Expression |
carcinoembryonic antigen (CEA); ICAM-1; MHC class I positive; MHC Class II (HLA DR, DQ, DP) negative |
| Genes Expressed |
transforming growth factor beta 1 (TGF, 189 pg per 106 cells per 24 hours) |
| Cellular Products |
transforming growth factor beta 1 (TGF, 189 pg per 106 cells per 24 hours) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms tumors in nude mice |
| Comments |
The LS411 cell line was found to be contaminated with mycoplasma, and was passed through nude mice to clear the cells of mycoplasma.
A culture submitted to the ATCC in September 1994 was found to be contaminated with mycoplasma, and progeny were cured by a 21 day treatment with BM Cycline.
Approximately 20% of LS411N cells express surface carcinoembryonic antigen (CEA).
The cells express low levels of ICAM-1 HLA class I antigen beta-2-microglobulin.
The cells secrete low levels of latent TGF-beta 1.
TGF-beta 1 and TGF beta 2 are do not inhibit the proliferation of LS411N cells.
Colony forming efficiency was 25% in methylcellulose medium. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C
Subculture Ratio: 1:3
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation |
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Population Doubling Time |
24 hrs |
| Name of Depositor |
L Suardet |
| Deposited As |
Homo sapiens |
| Passage History |
The cells obtained after passage in nude mice are referred to as LS411N cells. |
| Year of Origin |
1985 |
| References |
Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643
|
